In both cases, red wines must be decolourised (Iland et al. In the Lane and Eynon method, standard glucose solution is used as titrant, but in the Rebelein method, the concentration of Cu 2+ is determined by reacting with excess iodine and then estimating the remaining iodine by titrating with standard thiosulfate solution. These are the (i) Lane and Eynon and (ii) Rebelein methods, both of which rely on reacting the sugars with alkaline cupric tartrate and then titrating to determine the excess copper ions (Cu 2+). Space required: Minimal bench space Reaction/titrationĭescription: There are two procedures commonly used to estimate sugars in wines using this technique. The kits supply instructions and colour charts to assist in interpretation of results. The product is used to determine the amount of reducing substances (glucose and fructose) in wine. Commercially available test kits (such as Clinitest ® or OenoCheck ®) use a reagent tablet based on the Benedict’s copper reduction reaction, combining reactive ingredients with an integral heat generating system. Clinitest ® or OenoCheck ® (rapid method using test pills)ĭescription: This rapid technique is useful to establish the approximate sugar content of wines in the range 0.1-2.0% (i.e. There are several techniques of varying degree of difficulty and analytical accuracy that are used for this purpose. This page provides a brief summary of the procedures and equipment requirements for some commonly used techniques for determination of the concentration of residual sugar (predominantly glucose and fructose) in wines. by high performance liquid chromatography (HPLC).
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